MCQ for Centrifugation

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Question 1: Differential centrifugation is based on the differences in ______ of biological particles of different ___________.
  • INCORRECT. Please try again.
  • CORRECT. Differential centrifugation is based on the differences in the sedimentation rate of biological particles of different sizes and density.
  • INCORRECT! Please try again.
  • INCORRECT! Please try again.
Question 2: Which of the following statements about the basic principle of sedimentation is False?
  • INCORRECT. The statement is true.
  • CORRECT. The statement is false because the more massive a biological particle is, the faster it moves in a centrifugal field.
  • INCORRECT. The statement is true.
  • INCORRECT. The statement is true.
Question 3: Which of the following is/are the application of analytical centrifugation?

  • i. the determination of the purity of macromolecules.
  • ii. the determination of the relative molecular masses of solutes in their native state.
  • iii. the examination of changes in the molecular mass of supramolecular complexes.
  • iv. the detection of conformational changes.

  • INCORRECT. It is an application of analytical centrifugation, but not the only one.
  • INCORRECT. They are two of the application of analytical centrifugation, but not the only two.
  • INCORRECT. They are three of the application of analytical centrifugation, but not the only three.
  • CORRECT. All of these are application of analytical centrifugation.
Question 4: Which of the following is/are appropriate care and maintenance of centrifuges?

  • i. Proper balancing of centrifuge tubes.
  • ii. Avoid long time exposure of the rotors to alkaline solution and acidic buffers.
  • iii. Cleaning centrifuges and rotors with coarse brushes and then wash them with distilled or deionised water after every run.
  • iv. For overnight storage, the rotors are left upside down and then positioned in a safe and dry place.

  • INCORRECT. One of them is wrong.
  • INCORRECT. One of them is wrong.
  • CORRECT. Only option i, ii and iv are appropriate care and maintenance of centrifuges. However, cleaning centrifuges and rotors with coarse brushes may cause damage to the protective layers of rotors. Therefore, option iii is wrong.
  • INCORRECT. One of them is wrong.
Question 5: After centrifugation of milk, the supernatant is
  • INCORRECT. Please try again.
  • CORRECT. After centrifugation of milk, the supernatant is whey.
  • INCORRECT. After centrifugation of milk, casein is at the bottom of the tube.
  • INCORRECT. Please try again.