MCQ for ELIZA (Enzyme-linked immunosorbent assay)

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Question 1: ELISA (enzyme-linked immunosorbent assay) allows for rapid screening and quantification of the presence of _______ in a sample.
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  • CORRECT! ELISA (enzyme-linked immunosorbent assay) allows for rapid screening and quantification of the presence of antigen in a sample.
  • INCORRECT. Please try again.
Question 2: Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order
i. incubate with antibody-enzyme complex that binds primary antibody
ii. coat surface with antigen, block unoccupied sites with nonspecific protein
iii. add substrate, formation of colored product indicates presence of specific antigen
iv. incubate with primary antibody against specific antigen
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  • INCORRECT! Please try again.
  • INCORRECT! Please try again.
  • CORRECT! The steps for ELIZA are 1. coat surface with antigen, block unoccupied sites with nonspecific protein. 2. incubate with primary antibody against specific antigen. 3. incubate with antibody-enzyme complex that binds primary antibody. 4. add substrate, formation of colored product indicates presence of specific antigen.
Question 3: What is the role of goat anti-rabbit IgG horseradish peroxidase conjugate in the experiment?
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  • CORRECT! Goat anti-rabbit IgG horseradish peroxidase conjugate is the second antibody in the experiment.
  • INCORRECT. Please try again.
Question 4: In order to stop the reaction between enzyme and substrate, what process is needed?
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  • CORRECT! In the experiment, strong acid H2SO4 is added to sample to stop the enzyme reaction.
  • INCORRECT. Please try again.
  • INCORRECT! Blocking solution is added so as to block the remaining protein-binding sites on well. But it is not for stopping the enzyme reaction. Please try again.
Question 5: In the experiment, product formation (monitored as color intensity) is _________ the concentration of the antigen (BSA) solution in the sample.
  • CORRECT! Product formation (monitored as color intensity) is proportional to the concentration of the protein of interest in the sample.
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  • INCORRECT! One of them is correct. Please try again.