MCQ for Gel filtration chromatography

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Question 1: For gel filtration chromatography of proteins, which of the following is True?
  • INCORRECT. The statement is false. Large or elongated proteins cannot enter the pores in the beads.
  • CORRECT. The statement is true. Smaller proteins enter the pores in the beads.
  • INCORRECT. The statement is false. Large or elongated protein elute from the bottom of the column first.
  • INCORRECT. The statement is false. Smaller proteins elute from the bottom of the column later.
Question 2: For gel filtration column chromatography, which two of the following has linear relationship?
  • i. Amount of protein.
  • ii. Relative elution volume of a protein (void volume over elution volume of a protein.
  • iii. Logarithm of protein molecular mass.
  • INCORRECT. Not these two. Please try again.
  • CORRECT. There is a linear relationship between the relative elution volume of a protein and the logarithm of its molecular mass.
  • INCORRECT. Not these two. Please try again.
  • INCORRECT. Two of them have linear relationship.
Question 3: Which of the following are stationary phases commonly used for the gel filtration column?

  • i. Dextran
  • ii. Agarose
  • iii. Silica

  • INCORRECT. Please try again.
  • INCORRECT. Please try again.
  • INCORRECT. Please try again.
  • CORRECT. The stationary phases for gel filtration are generally based on silica, polymethacrylate or polyvinyl acetate or chloride or on cross-linked dextran or agarose.
Question 4: Which of the following is/are another term of gel filtration?

  • i. Desalting
  • ii. Size exclusion chromatography
  • iii. Permeation chromatography
  • iv. Molecular sieve chromatography

  • INCORRECT. More should be chosen.
  • INCORRECT. More should be chosen.
  • INCORRECT. More should be chosen.
  • CORRECT. These three terms can also describe gel filtration except desalting. Desalting is an application of gel filtration.
Question 5: Which of the following is not the application of gel filtration?
  • INCORRECT. The main application of gel filtration is in the purification of biological macromolecules through size separation.
  • INCORRECT. It is an application of gel filtration. A plot of the logarithm of the relative molecular mass of individual known proteins versus their retention volume gives a linear standard curve, from which relative molecular mass of an unknown protein with a known retention volume can be deduced.
  • INCORRECT. It is an application of gel filtration. Protein in solution can be concentrated by the addition of dry SephadexG-25 (coarse). The swelling gel absorbs water and low molecular mass substances, whereas high molecular weight protein remains in solution. After some time, the gel is removed by centrifugation, leaving a concentrated protein.
  • CORRECT. Purification, relative molecular mass determination and protein concentration are applications of gel filtration.