MCQ for Bacterial transformation

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Question 1: Which of the following will be taken up by cell in the process of genetic transformation?
i. chromosome
ii. plasmid
iii. damaged DNA
iv. naked DNA
  • INCORRECT. Please try again.
  • ONE IS MISSING! Please try again.
  • CORRECT! All three types of small DNA can be taken up by cell in the process of genetic transformation.
  • INCORRECT. Please try again.
Question 2: In the experiment, what kind of stress is used to induce bacterial transformation?
  • INCORRECT! Please try again.
  • INCORRECT! Please try again.
  • CORRECT! In the experiment, micro-tubes are placed on ice for 10 minutes, then to a 42oC air bath for 2 minutes (heat shock), and then back onto ice for 5 minutes (cold shock).
  • INCORRECT! Please try again.
Question 3: In the experiment, “BamHI/NotI” digested plasmid DNA is used. What are BamHI and NotI? They are also called _________.
I. DNA restriction enzymes.
II. DNA ligases.
III. Restriction endonucleases.
  • INCORRECT! One of them is wrong. Please try again.
  • INCORRECT! One of them is wrong. Please try again.
  • CORRECT! Restriction enzymes are enzymes from bacteria that can recognize specific base sequences in DNA and cut the DNA at specific site. They are also called restriction endonucleases.
  • INCORRECT! One of them is wrong. Please try again.
Question 4: 4. Restriction enzymes are capable of _______ DNA fragments of interest. The DNA to which the fragment of interest is attached to may be a vector. Common vectors include plasmids, which can replicate in the host cells. _______ completes the joining of the fragment to the vector.
  • INCORRECT. Please try again.
  • CORRECT! Restriction enzymes are for removing DNA fragments of interest. DNA ligase joins the fragment to the vector by phosphodiester linkage.
  • INCORRECT! Helicase is for unwinding and separating DNA strands. But it is not for joining DNA fragments. Please try again.
  • INCORRECT! Helicase is for unwinding and separating DNA strands. But it is not for joining DNA fragments. Please try again.
Question 5: Which of the following are the prerequisites for introducing and confirming foreign DNA into the recipient bacterial cells?
I. preparation of competent cells
II. transformation of competent cells
III. selection of transformants
  • INCORRECT! Please try again.
  • INCORRECT! Please try again.
  • INCORRECT! Please try again.
  • CORRECT! The process includes the preparation of competent cells, transformation of competent cells and selection of transformants.