MCQ for DNA sequencing

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Question 1: For analyzing DNA sequences, two techniques have been developed, one is _________ method frequently termed_________, involving the enzymatic synthesis of DNA and stopping the synthesis at specific nucleotides. While another technique, the __________ method of DNA sequencing developed by ___________ is often used for sequencing small fragments of DNA.
  • INCORRECT! Please try again.
  • INCORRECT! Please try again.
  • CORRECT! For analyzing DNA sequences, two techniques have been developed, one is enzymatic method frequently termed Sanger sequencing, involving the enzymatic synthesis of DNA and stopping the synthesis at specific nucleotides. While another technique, the chemical cleavage method of DNA sequencing developed by Maxam and Gilbert is often used for sequencing small fragments of DNA.
  • INCORRECT! Please try again.
Question 2: Which of the following is(are) the advantage(s) of using capillary electrophoresis in Sanger sequencing, for detection of sequenced DNA?
I. Thin capillary tubes are used to obviate the need to pour sequencing gels and it requires little manual operation
II. The electrophoretic run time is longer
III. High throughput can be achieved
  • INCORRECT! One of them is wrong. Please try again.
  • INCORRECT! One of them is wrong. Please try again.
  • CORRECT! The advantages of using capillary electrophoresis are: the thin capillary tubes are used to obviate the need to pour sequencing gels and it requires little manual operation. This substantially reduces the electrophoretic run time and allows high throughput sequencing.
  • INCORRECT! One of them is wrong. Please try again.
Question 3: In the experiment, which of the following is Not used for the preparation of reaction mixtures in a PCR tube?

  • INCORRECT! Terminator ready reaction mix is used for the preparation of reaction mixture in a PCR tube. Please try again.
  • INCORRECT! Primer is used for the preparation of reaction mixture in a PCR tube. Please try again.
  • INCORRECT! Plasmid DNA is used for the preparation of reaction mixture in a PCR tube. Please try again.
  • CORRECT! EDTA is not used for the preparation of reaction mixture in a PCR tube.
Question 4: What is the purpose of adding Formamide into the tube in the experiment?
  • INCORRECT! Please try again.
  • CORRECT! Adding Formamide into tube is to prevent reannealing of DNA.
  • INCORRECT! Please try again.
  • INCORRECT! Please try again.
Question 5: In the experiment, DNA is finally recovered by precipitation of the supernatant with________?
  • INCORRECT! Please try again.
  • CORRECT! DNA is finally recovered by precipitation of the supernatant with ethanol.
  • INCORRECT! Please try again.
  • INCORRECT. Please try again.