MCQ for Isolation of DNA

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Question 1: What kind of cells is used for extraction of DNA in the experiment?
  • INCORRECT. It is costly and invasive method is needed to obtain the cells.
  • INCORRECT. Please try again.
  • INCORRECT. It is costly and invasive method is needed to obtain the cells.
  • CORRECT. It is simple and the gentlest method for obtaining the cells.
Question 2: DNA extraction requires _________to lyse the epithelial cells and to degrade compounds inhibitory to amplification.
  • INCORRECT. Please try again.
  • CORRECT. Heat treatment is required to lyse epithelial cells and to degrade compounds inhibitory to amplification.
  • INCORRECT. Please try again.
  • INCORRECT. Mechanical shearing should be prevented as it causes DNA fragmentation.
Question 3: After centrifugation, the supernatant being pipetted out contains ____

  • INCORRECT. Please try again.
  • CORRECT. The supernatant is DNA extracted from cells.
  • INCORRECT. Cellular debris is at the bottom of the tube.
  • INCORRECT. Only one of them is the correct answer.
Question 4: In DNA extraction, ________ is included for chelating the _____ ions needed for enzymes ______to prevent degradation of DNA.
  • INCORRECT. Ribonuclease (RNase) catalyzes the degradation of RNA, but not DNA
  • CORRECT. EDTA chelates Mg2+, which is needed by deoxyribonuclease (DNase) to degrade DNA.
  • INCORRECT. Enzyme will not chelate cations for such purpose.
  • INCORRECT. EDTA will not chelate Ca2+ for such purpose.
Question 5: Which of the following is not a method to remove contaminant protein from sample solution?
  • INCORRECT. It is a method to remove contaminant protein.
  • INCORRECT. It is a method to remove contaminant protein.
  • INCORRECT. It is a method to remove contaminant protein.
  • CORRECT. Washing with buffer containing EDTA will not remove protein from sample solution. It is a method to dissolve the DNA pellet.