Virtual Lab on biochemistry and biotechnology techniques for NSS

Question 1: Which of the following is FALSE?

Restriction enzymes cut DNA of specific species.
CORRECT! Restriction enzymes can cut DNA at specific sequence of any species
DNA fragments cut by a restriction enzyme can pair with the complementary DNA of any species.
INCORRECT! This statement is true
Some restriction enzymes have more than one restriction site.
INCORRECT! This statement is true
The use of restriction enzyme in bacteria is to digest DNA of invading bacterial virus.
INCORRECT! This statement is true

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Question 2: Which of the following comparison between restriction enzyme EcoRV and protease Pepsin is CORRECT?

They recognize and cut specific sequence of DNA or polypeptides.
INCORRECT! Pepsin cut non-specifically within polypeptide
They are extracted from bacteria.
INCORRECT! Pepsin is produced in human
They work by breaking the bonding between nucleotides or amino acids.
CORRECT! Restriction enzymes work by hydrolyzing phosphodiester bond between nucleotides in DNA
The enzymatic reactions generate amino acids or nucleotides.
INCORRECT! Restriction enzymes only cut DNA strand, without breaking down it into simple subunits

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Question 3: According to information in the following table, which combination of enzymes leaves ends that can be ligated together?

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Question 4: If a bacterium contains no restriction enzyme, which of the following would you expect to happen?

It cannot replicate its DNA.
INCORRECT! Restriction enzyme is not involved in DNA replication
It cannot synthesize its own protein.
INCORRECT! Restriction enzyme is not involved in protein synthesis
It will be easily infected and lysed by bacteriophages.
CORRECT! The natural function of restriction enzyme in bacteria is to digest the DNA of invading bacteriophages
All of the above.
INCORRECT! Please try again!

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Question 5: Restriction enzymes cleave nucleic acids at specific site by

breaking the hydrogen bonds between bases.
INCORRECT! This is for the separation of double stranded DNA
hydrolyzing the phosphodiester bond of DNA.
CORRECT!
condensing the phosphodiester bond of DNA.
INCORRECT! Restriction enzymes work on the phosphodiester bond by a different reaction.
breaking the covalent bond between the base and the sugar nucleic acid.
INCORRECT! Restriction enzymes do not work on this bond

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Revision Questions : Work of a restriction enzyme