Revision Questions : Recombinant DNA technology

Status Bar
Question 1:To synthesize a protein using recombinant DNA technology, it must involves:
    1. Cutting of a desired gene by restriction enzymes.
    2. Culturing bacteria for synthesizing the recombinant proteins.
    3. Inserting the recombinant DNA into a living organism.
  • INCORRECT! Yeast and other organisms can also be used to synthesize recombinant proteins.
  • CORRECT! Yeast and other organisms can also be used to synthesize recombinant proteins.
  • INCORRECT! Yeast and other organisms can also be used to synthesize recombinant proteins.
  • INCORRECT! Yeast and other organisms can also be used to synthesize recombinant proteins.
Question 2:Which of the following doesn’t match with its function in recombinant DNA technology?
  • INCORRECT! This is a correct match.
  • CORRECT! DNA ligase is for ligation of gene into plasmid.
  • INCORRECT! This is a correct match.
  • INCORRECT! This is a correct match.
Question 3:To insert a foreign DNA into a plasmid, they must
  • INCORRECT! They can have different number of bases.
  • IINCORRECT! They usually come from different types of cells.
  • CORRECT! Same restriction site allows them to be joined together.
  • INCORRECT! This is not necessary.
Question 4:Which of the following enzymes are needed for producing recombinant DNA from a target gene and a vector?
    1. DNA polymerase
    2. Restriction Endonuclease
    3. Ligase
  • INCORRECT! DNA polymerase is not needed for producing recombinant DNA
  • INCORRECT! DNA polymerase is not needed for producing recombinant DNA
  • CORRECT!
  • INOCRRECT! DNA polymerase is not needed for producing recombinant DNA
Question 5: Which of the following is a part of cloning recombinant DNA in bacteria?
    1. Cutting of cellular and plasmid DNA by the same restriction endonuclease.
    2. Mixing DNA ligase with digested cellular DNA and plasmid DNA.
    3. Select the transformed bacteria by electrophoresis.
  • INCORRECT! There are more than one correct answers
  • CORRECT!
  • INCORRECT! Transformed bacteria are usually selected by antibiotics on agar plate
  • INCORRECT! Transformed bacteria are usually selected by antibiotics on agar plate