Samples' Criteria Sample

Amount

• It is recommended that 25 to 200 pmole of protein be submitted.
• More sample provided greater the number and accuracy of amino acids identified. In general, more sample is required when submitted on PVDF than in solution.

Sample Form

• Sample can be prepared as solution, dried sample or electroblotted protein on PVDF membrane.
• For solution samples, solvent used in dissolving samples should contain only volatile components such as water, acetonitrile etc. Please concentrate your sample in a small volume of solution. Usually, no more than 60 μl of the sample will be loaded for sequencing.
• For proteins adsorbed onto PVDF, the piece of PVDF should be no larger than 3 x 7 mm and the number of pieces no more than five. Keep in mind that transfer is not 100% efficient so load an adequate amount of protein or peptide onto the gel prior to electrophoresis.
• Please note that nitrocellulose cannot be used in our sequencer.
• If your sample is supplied dried, we will try to dissolve it into distilled water. Insoluble samples will not be sequenced.

Purity

• Sample should contain one protein component only.
• Sample should be as homogeneous as possible and free of salts, detergents, lipids and other non-volatile ingredients (SDS, Tris etc.). Please state the possible contaminants in the submission form.